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Supernatants (200 L) were collected and protein concentration of cell lysates measured with a Coomassiebased BioRad kit (BioRad Laboratories, Hercules, CA). Increasing protein concentrations of cell lysates (triplicates in 50 L) were added to 96well plates coated with antiCyp immunoglobulin G (IgG) and incubated for 2 hours at 4°C.14th European congress on digital pathology J Pathol Johan Lundin 1,2, Nina Linder 3 1 Institute for Molecular Medicine Finland FIMM, Helsinki, Finland, 2 Department of Public Health Sciences, Karolinska Institutet, Solna, Sweden, 3 Institute for Molecular Medicine Finland FIMM and Uppsala University, Helsinki, Finland. E [email protected] The 14 th European Congress on Digital Pathology (ECDP 2018), was organized May 29 4th ISTM Annual Conference, TRANSMEDCON 2015, KolkataThe test was done on 4 ml of EDTA blood sample after obtaining informed Plasma was separated and stored at 80 degree C and was tested using the PAK 2LE kit which is a qualitative solid phase enzyme linked immunosorbent assay (ELISA) designed to detect IgG antibodies to HLA class I antigens and to epitopes on the platelet glycoproteins

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